Sakura was born and raised in the San Francisco Bay Area. Her mother is of Japanese origin and her father is Irish. She loved recreating characters and doing funny voices from a very young age. As a child, she begged her parents to help her get an agent, and then at the age of 14, she moved to Los Angeles to pursue acting as a career. Nichole attended Santa Susana High School in Simi Valley. Nichole went on to attend the University of Southern California as a Theater major and graduated early, after three years. She says it was a challenge for her to get cast in the university's own plays because she dedicated so much of her spare time to professional auditions outside of school.
- Nichole Sakura's stand-in is Holly Holstein.